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Open Access Highly Accessed Methodology article

Digital PCR provides sensitive and absolute calibration for high throughput sequencing

Richard A White, Paul C Blainey, H Christina Fan and Stephen R Quake*

Author Affiliations

Department of Bioengineering at Stanford University and Howard Hughes Medical Institute, Stanford, California 94305, USA

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BMC Genomics 2009, 10:116  doi:10.1186/1471-2164-10-116

Published: 19 March 2009

Additional files

Additional file 1:

dPCR analysis of mock library control. The figure shows the absence of digital counts from a mock sequencing library preparation (454). False-color image of 12.765 digital array at assay endpoint. Each grid point corresponds to a nanoliter-scale PCR reaction, with yellow squares revealing amplification due to the presence of at least one sequencing library template molecule. The panels show dilution series (indicated) of a library preparation carried out as usual but for omission of sample DNA. The Ace sample (described in Table 2 of the main text) is used here as a positive control.

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Additional file 2:

replicate quantification of 12 test libraries by UT-dPCR and UT-qPCR. The table shows data from the replicate quantification of 12 test libraries by UT-dPCR and UT-qPCR.

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Open Data