Digital PCR provides sensitive and absolute calibration for high throughput sequencing
- Equal contributors
Department of Bioengineering at Stanford University and Howard Hughes Medical Institute, Stanford, California 94305, USA
BMC Genomics 2009, 10:116 doi:10.1186/1471-2164-10-116Published: 19 March 2009
Additional file 1:
dPCR analysis of mock library control. The figure shows the absence of digital counts from a mock sequencing library preparation (454). False-color image of 12.765 digital array at assay endpoint. Each grid point corresponds to a nanoliter-scale PCR reaction, with yellow squares revealing amplification due to the presence of at least one sequencing library template molecule. The panels show dilution series (indicated) of a library preparation carried out as usual but for omission of sample DNA. The Ace sample (described in Table 2 of the main text) is used here as a positive control.
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