Table 2 |
|||
|
Primer sequences. |
|||
|
Name |
Position |
Direction |
Sequence 5'-3' |
|
|
|||
|
ML5'-1 |
5'-UTR |
Forward |
GACTGCCCGTGGCCCCATTATTTG |
|
ML1F |
5'-UTR |
Forward |
GCTTTAAGACCGGTTGGATCTAC |
|
SJ93 |
Exon 1 |
Forward |
GGGGAGGAAGCTGGATCTCTC |
|
RACE4F |
Exon1 |
Forward |
ATGGGGAGGAAGCTGGATCTCTCCAAG |
|
RACE2R |
Exon 1 |
Reverse |
ACCTCCCAGACATGCTTGGCCTCCTC |
|
RFLP-F1 |
Intron 1 |
Reverse |
ACACTGACACAGCTGATAACATCAC |
|
MV2R |
Exon 2 |
Forward |
GAAGTGCAAGATAGACCAGGAAAG |
|
RACE1R |
Exon 2 |
Reverse |
GCTTGCTGTTCAGCAGGAACTTGAAGG |
|
MV18F |
Exon 3 |
Forward |
GAAGGGGAATTCAGCTCTTCTAGG |
|
ML14F |
Exon 5–6 |
Forward |
CAGAGTGCTACAGCAGAATGC |
|
BlotE91F |
Exon 8–9 |
Forward |
CTCGGGAAGTCAGATTCATGAGC |
|
ML3R |
Exon 10 |
Reverse |
CTTTCTCTTCAACTCCTCCTCCTC |
|
ML16F |
Exon 12 |
Forward |
GTCTACCTGACTGCTGGGAAGG |
|
RACE5F |
Exon 15 |
Forward |
GCCACAGGTCCTCAGGAGGAGGTTC |
|
MV8R |
Exon 16 |
Forward |
ATGACTCCTTTGACCGCAACTC |
|
MV6F |
Exon 17 |
Reverse |
AGGACAGGTGGGTCATCACTG |
|
ML15R |
Exon 17 |
Reverse |
CTCAGGACAGGTGGGTCATCACTG |
|
ML3'-1 |
3'-UTR |
Reverse |
GATGAGGCAGGGTGGCAGGACCAG |
|
|
|||
|
Only base pair position 103, causing the R35W substitution, was shared by all the lavender phenotypes studied and those that were expected to be heterozygous at this locus. |
|||
|
Vaez et al. BMC Genetics 2008 9:7 doi:10.1186/1471-2156-9-7 |
|||
