PCR-RFLP analysis for the presence/absence of the LAV*L allele. PCR amplification from genomic DNA results in a band of size 480 bp. Digestion with BsrBI cuts the wild type LAV*N allele into two fragments of sizes 334 bp and 146 bp, while the LAV*L allele does not digest; (A) LAV*L/LAV*L, (B) LAV*N/LAV*L, (C) LAV*N/LAV*N.
Vaez et al. BMC Genetics 2008 9:7 doi:10.1186/1471-2156-9-7