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Open Access Methodology article

A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae

Robert J Brownlow12*, Deborah A Dawson1, Gavin J Horsburgh1, James J Bell3 and John D Fish2

Author Affiliations

1 NERC Molecular Genetics Facility, Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

2 Institute of Biological Sciences, Aberystwyth University, Wales, SY23 3DA, UK

3 Centre for Marine Environmental and Economic Research, School of Biological Sciences, Victoria University of Wellington, Po Box 600, Wellington, New Zealand

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BMC Genetics 2008, 9:55  doi:10.1186/1471-2156-9-55

Published: 19 August 2008

Additional files

Additional file 1:

Review of heterozygosity in microsatellite studies of marine and freshwater gastropods.

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Open Data

Additional file 2:

Characterisation of seven polymorphic microsatellite loci (Primer set_A) for Hydrobia ulvae.

Format: PDF Size: 91KB Download file

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Open Data

Additional file 3:

Characterisation of redesigned Primer set_B for Hydrobia ulvae.

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Open Data

Additional file 4:

Comparison of microsatellite genotyping data using two primer sets (A and B) for Hydrobia ulvae.

Format: PDF Size: 75KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data