|
Genes with synthetic slow growth or synthetic lethal interactions with tra1SRR3413 |
|||||||
| GENE1 |
Orf |
SSL2 |
GO3 |
Location, component4 |
phenotype5 |
stress6 |
#7 |
|
|
|||||||
| MCK1 |
YNL307C |
SL |
1, 3 |
cytoplasm, nucleus |
X |
28 |
|
| CDC73 |
YLR418C |
s |
1, 3, 10 |
Cdc73/Paf complex, nucleus |
T, R |
X |
159 |
| CSE2 |
YNR010W |
S |
1, 3, 10 |
mediator complex |
R |
X |
19 |
| HTZ1 |
YOL012C |
S |
1, 3, 10 |
nucleus |
186 |
||
| LEO1 |
YOR123C |
s |
1, 3, 10 |
Cdc73/Paf complex, nucleus |
T |
39 |
|
| RAD52 |
YML032C |
s |
1, 3, 10 |
nucleus |
X |
196 |
|
| SPT8 |
YLR055C |
s |
1, 3, 10 |
SAGA complex, nucleus |
62 |
||
| SRB2 |
YHR041C |
s |
1, 3, 10 |
Srb-mediator complex |
T, E |
34 |
|
| SWR1 |
YDR334W |
s |
1, 3, 10 |
SWR1 complex, nucleus |
R |
112 |
|
| VPS71 |
YML041C |
s |
1, 3, 10, 9 |
SWR1 complex, nucleus |
E |
97 |
|
| VPS72 |
YDR485C |
s |
1, 3, 10, 9 |
SWR1 complex, nucleus |
E |
87 |
|
| KAR3 |
YPR141C |
s |
1, 3, 7 |
microtubule, spindle pole body |
E |
131 |
|
| UBI4 |
YLL039C |
s |
1, 3, 8, 9 |
cytoplasm |
X |
7 |
|
| UBP15 |
YMR304W |
s |
1, 3, 8, 9 |
cytoplasm |
0 |
||
| SWC3 |
YAL011W |
s |
1, 3, 9, 10 |
SWR1 complex, nucleus, mitochondrion |
85 |
||
| DOA1 |
YKL213C |
s |
1, 9, 8 |
cytoplasm, nucleus |
X |
26 |
|
| TOR1 |
YJR066W |
S |
1,2,3,4,5,6,7,8,9,10 |
cellular membranes |
R |
X |
27 |
| CKB2 |
YOR039W |
s |
1,2,3,4,5,7,8,9,10 |
protein kinase CK2, CURI complex |
X |
14 |
|
| SNF1 |
YDR477W |
s |
1,3,4,5,6,7,8,9,10 |
cytoplasm, nucleus, nuclear evelope, mitochondrion, vacuole |
R |
X |
18 |
| SNF4 |
YGL115W |
S |
1,3,4,5,6,7,8,9,10 |
cytoplasm, nucleus, nuclear envelope, PM |
7 |
||
| CNB1 |
YKL190W |
s |
1,3,4,5,7,9,10 |
calcineurin complex |
X |
48 |
|
| EOS1 |
YNL080C |
S |
1,3,4,7 |
ER membrane |
X |
4 |
|
| SIS2 |
YKR072C |
s |
1,3,5,10 |
cytoplasm, nucleus |
X |
10 |
|
| NUM1 |
YDR150W |
s |
1,3,7,9 |
cell cortex, cellular bud tip, mitochondrion |
57 |
||
| SWI4 |
YER111C |
s |
1, 3, 7, 10 |
nucleus |
CW |
69 |
|
| UBP6 |
YFR010W |
S |
1,3,8,9,10 |
proteosome |
X |
28 |
|
| MSC1 |
YML128C |
s |
1,3,9,6 |
ER, mitochondrion |
R |
14 |
|
| ARP6 |
YLR085C |
s |
10, 1, 2 |
SWR1 complex, cytoplasm |
R |
149 |
|
|
|
|||||||
| CAF40 |
YNL288W |
s |
2, 10 |
CCR4-NOT complex |
R |
8 |
|
| NST1 |
YNL091W |
s |
2, 10 |
cytoplasm |
X |
2 |
|
| PUB1 |
YNL016W |
s |
2, 10 |
cytoplasm, nucleus, hnRNP complex |
X |
5 |
|
| PIH1 |
YHR034C |
s |
2, 3, 8, 10 |
cytoplasm, nucleus, snRNP complex |
2 |
||
| HMT1 |
YBR034C |
s |
2, 4 10 |
nucleus |
4 |
||
| PML39 |
YML107C |
SL |
2, 4, 10 |
nuclear pore, ribosome |
4 |
||
| HCR1 |
YLR192C |
s |
2, 8 |
translation IF3 complex |
T |
X |
5 |
| PPQ1 |
YPL179W |
S |
2, 8 |
cytoplasm |
0 |
||
| TMA23 |
YMR269W |
s |
2, 8 |
nucleolus, ribosome |
5 |
||
| RRP6 |
YOR001W |
SL |
2, 8, 10 |
nuclear exosome (RNase complex) |
16 |
||
| SKY1 |
YMR216C |
s |
2,3,4,8,10 |
cytoplasm |
5 |
||
| STP1 |
YDR463W |
s |
2,4,7,8,10 |
nucleus |
2 |
||
|
|
|||||||
| KNS1 |
YLL019C |
SL |
3 |
X |
0 |
||
| BIM1 |
YER016W |
s |
3, 1, 7 |
cytoplasm, microtubule, kinetochore, spindle pole body |
282 |
||
| NBP2 |
YDR162C |
s |
3, 7 |
cytoplasm, nucleus |
CW, R |
X |
49 |
| PRB1 |
YEL060C |
s |
3, 8, 9 |
vacuole |
X |
3 |
|
| SEL1 |
YML013W |
s |
3, 8, 9 |
ubiquitin ligase complex, ER, mitochondrion |
3 |
||
| TPS2 |
YDR074W |
s |
3,5,7,8,10 |
mitochondrion |
X |
4 |
|
|
|
|||||||
| ITR1 |
YDR497C |
s |
4, 5, 7, 9 |
plasma membrane |
X |
9 |
|
| NEW1 |
YPL226W |
s |
4, 5, 9, 8 |
cytoplasm, mitochondrion |
8 |
||
| AQR2 |
YBR043C |
s |
4, 7 |
plasma membrane |
1 |
||
| GTR1 |
YML121W |
s |
4, 9 |
cytoplasm, endosome, nucleus, vacuole |
T, E, R |
5 |
|
| VAM10 |
YOR068C |
SL |
4, 9 |
vacuolar membrane |
11 |
||
|
|
|||||||
| SER1 |
YOR184W |
s |
5 |
cytoplasm |
1 |
||
| SER2 |
YGR208W |
s |
5 |
cytoplasm, nucleus |
5 |
||
| GCR2 |
YNL199C |
SL |
5, 10, 3 |
nucleus |
E |
30 |
|
| ARO1 |
YDR127W |
SL |
5, 6 |
cytoplasm |
E |
0 |
|
| ARO2 |
YGL148W |
SL |
5, 6 |
cytoplasm |
1 |
||
| ETR1 |
YBR026C |
s |
5, 6 |
mitochondrion |
0 |
||
| LIP2 |
YLR239C |
s |
5, 6 |
mitochondrion |
7 |
||
| LIP5 |
YOR196C |
S |
5, 6 |
mitochondrion |
2 |
||
| OAR1 |
YKL055C |
s |
5, 6 |
mitochondrion |
0 |
||
| ERG3 |
YLR056W |
s |
5, 9 |
ER |
19 |
||
|
|
|||||||
| MDM34 |
YGL219C |
S |
6 |
mitochondrial outer membrane |
E, R |
10 |
|
| KGD2 |
YDR148C |
S |
6, 5 |
mitochondrion |
0 |
||
| MDM10 |
YAL010C |
s |
6, 9 |
Mdm10/Mdm12/Mmm1 complex, mitochondrial outer membrane |
T, E |
11 |
|
|
|
|||||||
| BEM1 |
YBR200W |
S |
7, 3, 1 |
bud neck |
E |
33 |
|
| BEM4 |
YPL161C |
s |
7, 3, 1 |
cytoplasm, nucleus |
T, E |
X |
29 |
| Smi1 |
YGR229C |
s |
7, 3, 1 |
bud tip, nucleus |
T, E, CW, R |
111 |
|
| ROM2 |
YLR371W |
SL |
7, 3, 9 |
bud tip |
X |
16 |
|
| CSF1 |
YLR087C |
s |
7, 5, 6 |
mitochondrion |
CW |
24 |
|
| ECM30 |
YLR436C |
s |
7, 9 |
cytoplasm |
CW |
1 |
|
| KRE1 |
YNL322C |
s |
7, 9 |
cell wall |
64 |
||
| VIP1 |
YLR410W |
S |
7, 9 |
cytoplasm |
15 |
||
| TPM1 |
YNL079C |
S |
7,9,3 |
actin cable |
E, CW, R |
21 |
|
|
|
|||||||
| RPS27B |
YHR021C |
S |
8 |
ribosome |
T |
1 |
|
| RPN4 |
YDL020C |
S |
8, 10, 1, 3 |
nucleus, proteasome |
T, E |
X |
242 |
| YDJ1 |
YNL064C |
S |
8, 9, 6 |
cytosol, microsome |
R |
X |
27 |
|
|
|||||||
| CCZ1 |
YBR131W |
s |
9 |
late endosome, membrane |
E, R |
X |
6 |
| MON1 |
YGL124C |
s |
9 |
cytosol, vacuolar membrane |
12 |
||
| VAM3 |
YOR106W |
s |
9 |
vacuolar membrane |
R |
9 |
|
| VAM7 |
YGL212W |
s |
9 |
vacuolar membrane |
T, E, CW, R |
8 |
|
| PIB2 |
YGL023C |
s |
9, 1, 7 |
late endosome, mitochondrion |
R |
0 |
|
| GSG1 |
YDR108W |
s |
9, 3 |
TRAPP complex |
X |
11 |
|
| YDR049W |
s |
9, 3, 8 |
cytoplasm, mitochondrion |
E, R |
|||
| MEH1 |
YKR007W |
S |
9, 4, 5 |
cytosol, vacuolar membrane |
X |
2 |
|
| PMR1 |
YGL167C |
s |
9, 4, 7 |
Golgi membrane |
E |
41 |
|
| AUT7 |
YBL078C |
s |
9, 5 |
autophagic vacuole, cytosol |
X |
2 |
|
| CCW12 |
YLR110C |
s |
9, 7 |
cell wall |
CW |
13 |
|
| COG5 |
YNL051W |
S |
9, 7 |
Golgi |
14 |
||
| COG6 |
YNL041C |
S |
9, 7 |
Golgi |
17 |
||
| COG7 |
YGL005C |
S |
9, 7 |
Golgi |
12 |
||
| COG8 |
YML071C |
S |
9, 7 |
Golgi |
17 |
||
| DID2 |
YKR035W |
s |
9, 7 |
cytoplasm, late endosome |
2 |
||
| GYP1 |
YOR070C |
S |
9, 7 |
Golgi, mitochondrion |
R |
48 |
|
| MNN10 |
YDR245W |
s |
9, 7 |
α-1,6-mannosyltransferase complex |
E, R, CW |
19 |
|
| MON2 |
YNL297C |
s |
9, 7 |
cytosol, endosome |
R |
40 |
|
| RIC1 |
YLR039C |
S |
9, 7 |
Golgi, nucleus |
E |
159 |
|
| RUD3 |
YOR216C |
s |
9, 7 |
Golgi |
26 |
||
| SEC22 |
YLR268W |
s |
9, 7 |
ER, Golgi |
CW |
44 |
|
| TLG2 |
YOL018C |
s |
9, 7 |
early endosome, trans Golgi |
R |
12 |
|
| VPS1 |
YKR001C |
S |
9, 7 |
cytoplasm, membrane fraction |
28 |
||
| VPS17 |
YOR132W |
s |
9, 7 |
endosome, retromer complex |
11 |
||
| VPS38 |
YLR360W |
s |
9, 7 |
Vps34 complex |
R |
12 |
|
| VPS41 |
YDR080W |
s |
9, 7 |
HOPS complex, vacuolar membrane, endosome |
R |
7 |
|
| VPS53 |
YJL029C |
SL |
9, 7 |
GARP complex, Golgi, cytoplasm |
T, R |
10 |
|
| YPT6 |
YLR262C |
s |
9, 7 |
Golgi |
E, R |
181 |
|
| YPT7 |
YML001W |
s |
9, 7 |
vacuole, mitochondrion |
T, R |
6 |
|
| YLR111W |
s |
9, 7 |
CW |
0 |
|||
| GET1 |
YGL020C |
S |
9, 8 |
GET complex, ER, mitochondrion |
R |
X |
284 |
| SSE1 |
YPL106C |
SL |
9, 8 |
cytoplasm |
T |
15 |
|
| BTS1 |
YPL069C |
S |
9,5,6 |
mitochondrion |
CW |
16 |
|
| UBP3 |
YER151C |
s |
9,7,10,1,3 |
cytoplasm |
R |
32 |
|
|
|
|||||||
| YDL180W |
s |
vacuole |
0 |
||||
| YEL033W |
s |
T |
0 |
||||
| YOR235W |
s |
0 |
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|
1 Genes are placed according to related GO terms with relevant groupings separated by lines. 2 Phenotype of double mutants. SL, synthetic lethal; S, severe slow growth; s, slow growth (in SC media at 33.5°C). For those underlined the phenotype is most easily detected on YPD at 30C as the single mutant shows reduced growth on SC media 3 Gene ontology: (1) DNA replication/repair and chromosome stability, (2) RNA processing, (3) cell cycle/microtubule, (4) general transport, (5) metabolism, (6) mitochondria, (7) polarity/cell wall, (8) protein/ribosome biosynthesis, (9) secretion/protein trafficking, (10) transcription, (11) unknown 4 Cellular compartments and/or components as indicated in the Saccharomyces Genome Database 5 Relevant phenotypes of single mutants as described in the Saccharomyces Genome Database. E, sensitivity to ethanol; CW, sensitivity to calcofluor white; T, shortened telomeres; R, sensitivity to rapamycin 6 Annotated in the Saccharomyces Genome Database as involved in stress response or autophagy 7 Number of previously identified SSL interactions in the Saccharomyces Genome Database | |||||||
Hoke et al. BMC Genetics 2008 9:46 doi:10.1186/1471-2156-9-46 |
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