Figure 5.

Subcellular distribution of CGI-146 protein tagged with GFP. The CGI-146-GFP fusion protein localized in Golgi complex marked with fluorescein isothiocyanate (A). Red fluorescence of mitochondria were stained with the mitochondrial-specific dye, Mito-Tracker Red (B) and blue fluorescence of nuclei were stained with Hoechst33342 (C). The overlay images were produced by merging all three signals together (D). The control micrographs (E-H) showed the GFP in the cytoplasm. Bars represent 30 μM.