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Open Access Highly Accessed Research article

Multiple forms of atypical rearrangements generating supernumerary derivative chromosome 15

Nicholas J Wang1, Alexander S Parokonny2, Karen N Thatcher3, Jennette Driscoll2, Barbara M Malone2, Naghmeh Dorrani1, Marian Sigman4, Janine M LaSalle3 and N Carolyn Schanen256*

Author Affiliations

1 Department of Human Genetics, UCLA Geffen School of Medicine, Los Angeles, California, 90095, USA

2 Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware, 19803, USA

3 Dept. of Medical Microbiology and Immunology, University of California, Davis, California, 95616, USA

4 Neuropsychiatric Institute, UCLA Geffen School of Medicine, University of California, Los Angeles, California, 90095, USA

5 Department of Biological Sciences, University of Delaware, Newark, DE, 19716, USA

6 Department of Pediatrics, Thomas Jefferson University, Philadelphia, Pennsylvania, 19107, USA

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BMC Genetics 2008, 9:2  doi:10.1186/1471-2156-9-2

Published: 4 January 2008

Abstract

Background

Maternally-derived duplications that include the imprinted region on the proximal long arm of chromosome 15 underlie a complex neurobehavioral disorder characterized by cognitive impairment, seizures and a substantial risk for autism spectrum disorders[1]. The duplications most often take the form of a supernumerary pseudodicentric derivative chromosome 15 [der(15)] that has been called inverted duplication 15 or isodicentric 15 [idic(15)], although interstitial rearrangements also occur. Similar to the deletions found in most cases of Angelman and Prader Willi syndrome, the duplications appear to be mediated by unequal homologous recombination involving low copy repeats (LCR) that are found clustered in the region. Five recurrent breakpoints have been described in most cases of segmental aneuploidy of chromosome 15q11-q13 and previous studies have shown that most idic(15) chromosomes arise through BP3:BP3 or BP4:BP5 recombination events.

Results

Here we describe four duplication chromosomes that show evidence of atypical recombination events that involve regions outside the common breakpoints. Additionally, in one patient with a mosaic complex der(15), we examined homologous pairing of chromosome 15q11-q13 alleles by FISH in a region of frontal cortex, which identified mosaicism in this tissue and also demonstrated pairing of the signals from the der(15) and the normal homologues.

Conclusion

Involvement of atypical BP in the generation of idic(15) chromosomes can lead to considerable structural heterogeneity.