Additional file 2.Standard curves for qOLA_CNV and Pyro_CNV. A serial dilution from 0% to 100% duplicated copy vs normal copy (PCR-amplified and cloned) was used for the estimation. (a) A curve estimated using peak height values from qOLA_CNV (correlation coefficient = 0.999). (b) A curve estimated using peak area values from qOLA_CNV (correlation coefficient = 0.999). (c) A curve estimated using Pyro_CNV (correlation coefficient = 0.995). (d) A comparison of the three curves by root mean square (RMS) of the bias to reference values and standard deviations. The qOLA_CNV using peak height measurements is the most accurate and precise of the three. Format: JPEG Size: 126KB Download file Seo et al. BMC Genetics 2007 8:81 doi:10.1186/1471-2156-8-81 |