Genotype assignment using a combination of qOLA_CNV to analyze KIT copy numbers and Pyro_Splice to quantify KIT copies with spliced forms. (a) Genotype assignment of parental animals. As the Patch and Belt phenotypes were not presented in the F1 population, all Korean native pigs were assigned to i/i, and Landrace pigs were assigned to I1/I1, I1/I2 and I1/i by the clustering measurements. (b) Genotype assignment of F1 animals. (c) Electrophoregram in qOLA_CNV and pyrogram in Pyro_Splice as representative examples of the three genotypes in the F1 population. In qOLA_CNV, the ratio was (duplicated copy/total copy) = [G/(G + C)]. In the Pyro_Splice assay, the ratio was (spliced copy/total copy) = [A/( A + G )].
Seo et al. BMC Genetics 2007 8:81 doi:10.1186/1471-2156-8-81