Table 2

Quantitative RT-PCR conditions and gene-specific primer sequences.
Gene
 Quantitative PCR Primers
 Annealing temp.
 Reading temp.
Gigaxonin
 F: catcgtgactgttggtggag
R: tggcaatgctgtccatgtat
 62°C
 83°C
Complement C3
 F: ctggagcagtcaaggtctacg
R: gctcaatggccatgatgtact
 66°C
 84°C
Butyrylcholinesterase
 F: aacaccgatcctccaaacttc
R: tcgacattgttgagcacgtag
 66°C
 80°C
Sialyltransferase
 F: atacctcactccagcccactt
R: accatgtgctttaccaacagc
 66°C
 80°C
Fatty acid binding protein 5 (FABP5)
 F: agttcagcagctggaaggaag
R: tgaaccaatgcaccatctgta
 68°C
 83°C
ATP-binding cassette A6 (ABCA6)
 F: actcaccgtgaaggaaaacct
R: aagaccccaccttcttttcaa
 66°C
 84°C
Meltrin alpha
 F: agtcaactcagcgagtgcttc
R: ggcacttggtgtggatattgt
 66°C
 86°C
ATP-binding cassette B4 (ABCB4)
 F: ctcgatggtcaagaagcaaag
R: ttttgacctcctgagagctga
 66°C
 84°C
Acyl coenzyme A: cholesterol acyltransferase
 F: ctgattccagaagccactgag
R: ctcttctgaggcaccctcttt
 66°C
 85°C
Leptin
 F: ccaaaaccctcatcaagacaa
R: gctcttagagaaggccagcac
 66°C
 86°C

Leung et al. BMC Genetics 2007 8:6   doi:10.1186/1471-2156-8-6