Additional file 1.
Identification of Y-chromosome sequences; results from blast analysis and PCR screen.
a Name of shotgun sequence.
b GenBank accession number of shotgun sequence used as template for primer design.
c Best hit versus Human genome. Shown here is the highest-scoring alignment with a single region on the human genome.
d Female dog genome hit. A match versus the female dog genome (sequences sharing >95% nucleotide identity for more than 90% of their lengths with regions of the female dog genome sequence) is indicated with a "+" and a non-match is indicated with a "-".
e,f Result from the first (two males and two females) and second (three males and three females) PCR screen. "Candidate" indicates a sequence that gave a PCR fragment for male dogs only, "Not male specific" a sequence that gave a PCR fragment for both male and female dogs, a dash indicates a sequence that was not tested, "Approved" indicates a sequence that gave a PCR fragment for all five male dogs tested and no band for the five female dogs, and "Failed" indicates a sequence that did not give three male bands and/or gave female bands in the second test.
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Natanaelsson et al. BMC Genetics 2006 7:45 doi:10.1186/1471-2156-7-45