Figure 1.

Dlk1-Gtl2 domain and Gtl2lacZ integration. A) Schematic of the Dlk1-Gtl2 imprinted domain. Maternally expressed genes are shown in pink and paternally expressed genes in blue; the direction of their transcription is indicated. Black lollipops indicate the positions of the three known Dlk1-Gtl2 DMRs, which have been designated with individual letters, D, Dlk1 DMR, I, IG DMR, and G, Gtl2 DMR. The dotted arrow indicates a genomic region that produces multiple miRNAs and snoRNAs; emerging evidence suggests contiguous transcription through this region. B) Diagram of the Gtl2lacZ integration. The blue arrows represent the lacZ genes and the green arrows represent the Neo genes. The broken line represents an internal rearrangement that has been refractory to analysis; its size is estimated by Southern blotting. The gold box represents the Gtl2 DMR. C) Growth curves of Gtl2lacZPat¹²⁹ mice in comparison to wild type littermates (WT, n = 11, Gtl2lacZPat¹²⁹, n = 3). D) Growth curves of Gtl2lacZPat^B6 mice in comparison to wild type littermates (WT, n = 6, Gtl2lacZPat^B6, n = 7). E) Growth curves of Gtl2lacZMat¹²⁹ mice in comparison to wild type littermates (WT, n = 7, Gtl2lacZMat¹²⁹, n = 8). F) Growth curves of Gtl2lacZMat^B6 mice in comparison to wild type littermates (WT, n = 7, Gtl2lacZMat^B6, n = 8). In all graphs the solid line represents wild type animals and the dashed line represents Gtl2lacZ animals.