Table 3

ncl-1 versus GFP-LacI•lacO as markers for mosaic analysis.

Phenotype of cells


Cell

W, + or N,-

W, -

N, +

?


m2

32 (87%)

0

3 (8%)

2

m3L

31 (84%)

2 (5%)

4 (11%)

m3VL

31 (84%)

1 (3%)

5 (13%)

m4

28 (76%)

0

9 (24%)

m3R

31 (84%)

2 (5%)

4 (11%)

m3VR

30 (81%)

1 (3%)

6 (16%)

m3DR

29 (78%)

0

8 (22%)

m3DL

30 (81%)

0

7 (19%)

hyp7(dorsal head)

30 (81%)

1(3%)

6 (16%)

hyp7 (ventral head)

32 (87%)

0

5 (13%)

P/I

33 (89%)

1 (23%)

3 (8%)

P3.p

30 (81%)

1 (3%)

3 (8%)

3

P4.p

29 (78%)

2 (5%)

4 (11%)

2

P5.p

30 (81%)

2 (5%)

4 (11%)

1

P6.p

32 (87%)

0

4 (11%)

1

P7.p

33 (89%)

0

3 (8%)

1

P8.p

26 (70%)

1 (3%)

9 (24%)

1

B

22 (59%)

1 (3%)

14 (38%)

F

31 (84%)

1 (3%)

5 (13%)

U

29 (78%)

2 (5%)

6 (16%)

hyp7 (ventral tail)

29 (78%)

0

7 (19%)

1

hyp7 (anus)

21 (57%)

1 (3%)

8 (22%)

7


Mean %

80%

2%

16%

2%

Standard Dev

8%

2%

7%

4%


Mosaic animals from strain PS3047 (n = 37) were used to determine the occurrence of the Ncl phenotype and GFP-LacI•lacO. Scored by cell from anterior to posterior of the worm: m2, m3L, m3VL, m4, m3R, m3VR, m3DL, m3DR, hyp7(dorsal, head), hyp7(ventral head), P/I, P3.p, P4.p, P5.p, P6.p, P7.p, P8.p, B, F, U, hyp7(tail), hyp7(anus). +: GFP-LacI bound to the lacO, fluorescent spot; -: diffuse fluorescence, W: normal nucleolus (non-Ncl) size, N: enlarged nucleolus (Ncl) and ?: undetermined.

Gonzalez-Serricchio and Sternberg BMC Genetics 2006 7:36   doi:10.1186/1471-2156-7-36

Open Data