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Oligonucleotide primers used in this study. |
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| Primer name |
Nucleotide sequence (5'→3') |
Function |
|
|
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| Pk-5'-(-1056)-F† |
ACAGCCTGTGATGGGAATGAC |
a, b, e |
| Pk-5'-(-740)-R† |
TTGAGTGCTGACGCCCATCC |
b |
| Pk-5'-(-834)-F |
TGGGCACCCATTGAGTGCCA |
b, e |
| Pk-5'-(-550)-R |
ACCTCGCCCCATCTTCACAC |
e |
| Pk-5'-(550T)-F |
GAACAAATTACCAATAGCAATATGT |
e |
| Pk-5'-(-550insC)-R |
CCTCGCCCCATCTTCACAGC |
e |
| Pk-5'-(550insC)-F |
AACAAATTACCAATAGCAATATGCT |
e |
| Pk-5'-(-477)-F |
GCGGCGTTAAGGATACAGCAA |
b |
| Pk-5'-(-418)-R |
CTGATCCCACCGCCTCCTG |
b |
| Pk-5'-(-235)-F |
GCGCTCCCTACCTGTTGGC |
b |
| Pk-5'-(-131)-F |
GGACCGGGACCCGCAGGG |
a, b |
| Pk-5'-(-160Gmis)-R |
CCCGGTCCCCAGAGCACC |
e |
| Pk-5'-(-160Amis)-R |
CCCGGTCCCCAGAGCACT |
e |
| Pk-5'-(-160G)-R |
CCGGTCCCCAGAGCCCTC |
e |
| Pk-5'-(-160A)-R |
CCCGGTCCCCAGAGCCCTT |
e |
| Pk-int1-35-R |
CGTCCCCCGCAACATCGGC |
b |
| Pk-int1-160-R |
GCACAAATGTCGCCTCCAGAA |
a, b |
| Pk-ex2-74_75insC-F |
AGGTCGGCTGCTGAGCCCA |
e |
| Pk-int2-R |
GGGTGCAACCTGATTGCTAAG |
e |
| Pk-109G-F |
TTCACGTTTTTCGTCTCCATCG |
e |
| Pk-109A-F |
TTCACGTTTTTCGTCTCCATCA |
e |
| Pk-987G-F |
CACGCGGTTCGAGGCCACG |
e |
| Pk-987A-F |
CACGCGGTTCGAGGCCACA |
e |
| Pk-1120-R |
GGAAGGGCGGCCCAGTGC |
e |
| Pk-1006-F |
CCAGGGCACTGCTGGCCC |
c, d |
| Pk-1253-F |
GGACAGTGTCCTGTCTCGAG |
d |
| Pk-1697G-R |
CCTGTCTGAGGGAAGGGGC |
e |
| Pk-1697A-R |
CCCTGTCTGAGGGAAGGGGT |
e |
| Pk-1791-R |
TTATTCTATTGATTATTCTCCTGTG |
d |
| Pk-1881-R |
CCCCGTCAGAAGAATGGAGC |
c, d |
| HGH-F |
TGCCTTCCCAACCATTCCCTTA |
f |
| HGH-R |
CCACTCACGGATTTCTGTTGTGTTTC |
f |
| JK-781-L-F |
GCATGCTGCCATAGGATCATTGC |
f |
| JK-943-L-R |
GAGCCAGGAGGTGGGTTTGCC |
f |
| MO-21 |
GGTGAGAGAAGGAGGGTGAG |
f |
| MO-31 |
CCAGCACCCCGGCCAGCA |
f |
|
† F = Forward. R = Reverse. The underlined nucleotide is mismatched for better specificity. a. used for amplification of the 5' regulatory region. b. used for sequencing of the 5' regulatory region. c. used for amplification of the 3'-UTR. d. used for sequencing the 3'-UTR. e. used for PCR-ASP. f. used as internal control. | ||
Hellberg et al. BMC Genetics 2005 6:49 doi:10.1186/1471-2156-6-49 |
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