Table 2

ISSR primers useful for the amplification of the eight populations of A. acutifolius.

Primer Name

Sequence 5'-3'

AT (°C)


3

(CA)8AT

50

4

(CA)8AC

51.7

5

(CA)8GT

51.7

8

(CA)8GAC

54.7

15

GGTC(AC)7

56

16

CGTC(AC)7

56

17

CAGC(AC)7

56

21

CAGC(TC)7

56

23

GAG(TC)8

56

8082

(CT)9G

57

8564

(CAC)7T

58

8565

GT(CAC)7

58

BEC

(CA)7YC

54

CHR

(CA)7YG

54

DAT

(GA)7RC

54

HAD

CT(CCT)3CRC

54

MAN

CA(CCA)3CRC

54

OH

(GAG)7RG

66.7

TE

GT(GGT)3GRC

54

W7

(CT)8RG

52.8

W814

(CT)8TG

52

W844

(CT)8RC

52.8

W902

(GT)6AY

39


AT, annealing temperature.

Sica et al. BMC Genetics 2005 6:17   doi:10.1186/1471-2156-6-17

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