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Open Access Research article

Apolipoprotein C3 SstI polymorphism and triglyceride levels in Asian Indians

S Chhabra1, R Narang2, LR Krishnan3, S Vasisht3, DP Agarwal4, LM Srivastava5, SC Manchanda2 and N Das1*

Author Affiliations

1 Departments of Biochemistry, All India Institute of Medical Sciences, New Delhi-110029, India

2 Department of Cardiology, All India Institute of Medical Sciences, New Delhi-110029, India

3 Department of Cardiac Biochemistry, All India Institute of Medical Sciences, New Delhi-110029, India

4 Institute of Human Genetics, University of Hamburg, Germany

5 Department of Biochemistry, Sir Ganga Ram Hospital, New Delhi-110060, India

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BMC Genetics 2002, 3:9  doi:10.1186/1471-2156-3-9

Published: 6 June 2002

Abstract

Background

A close association between Sst I polymorphism in the 3' untranslated region of the apolipoproteinC3 (APOC3) gene and levels of plasma triglycerides (TG) had been reported by different investigators. Hypertriglyceridemia(HTG) is a known risk factor for coronary artery disease (CAD) in the context of Asian Indians. We conducted a study on the relationship between APOC3 SstI polymorphism (S1S1, S1S2 and S2S2 genotypes) and plasma TG levels in a group of 139 male healthy volunteers from Northern India.

Methods

DNA samples were analyzed by polymerase chain reaction (PCR) followed by SstI digestion. Digested PCR products were run on 3% agarose gel and visualized by ethidium bromide staining.

Results

Rare S2 allele was highly prevalent in our study population (0.313) as compared to the Caucasians (0.00–0.11). The genotypic distribution was in agreement with Hardy-Weinberg equilibrium. S2 allele was almost two times more prevalent in the HTG group (N = 34) as compared to NTG group (N = 105) (p = 0.001). Multiple logistic regression revealed S1S2 individuals had age-adjusted odds ratio of 2.43 (95%CI = 0.99–6.01, p = 0.054) and S2S2 had 9.9 (95%CI = 2.66–37.29, p = 0.0006) for developing HTG in comparison to S1S1 genotype.

Conclusions

Our study shows a significant association between rare S2 allele and HTG in Asian Indians.