Detection of mutations in the dystrophin gene via automated DHPLC screening and direct sequencing
1 Division of Genetics, Children's Hospital, Boston, Massachusetts, USA
2 Center for Human and Clinical Genetics, Leiden University Medical Center, Leiden, Nederland
3 Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
4 Department of Neurology, Children's Hospital, Boston, Massachusetts, USA
5 Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA
BMC Genetics 2001, 2:17 doi:10.1186/1471-2156-2-17Published: 17 October 2001
Additional file A: Primer sequences and DHPLC temperatures Excel spreadsheet:
This file contains a database that includes primer sequences for amplifying each of the dystrophin screening fragments, fragment length, and the suggested first pass and second pass WAVE® oven temperatures.
Format: XLS Size: 36KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file B: Details regarding procedure and cost of DHPLC screening, observations regarding the dystrophin gene, and statistics about DMD carrier females.:
This file outlines the expenditures and procedures related to DHPLC screening and comments upon interesting observations made as a result of this project.
Format: DOC Size: 30KB Download file
This file can be viewed with: Microsoft Word Viewer