Table 1

Population surveys of D negative blood donors documented D negative and screened by RHD PCR-SSP

Samples (n)


Documented phenotype

screened

PCR-SSP positive

D positive*


Testing as single samples

Ccddee

433

34

0

ccddEe

271

5

2

CCddee

24

4

0

CcddEe

19

4

2

ccddEE

6

1

0

CcddEE

1

0

0

ccddee

314

0

0

Testing as pools of 20 samples

ccddee

7,374

2

1

Total

50

5


*Samples uncovered on further analysis as weak D, partial D or D+/- chimera. Positive for at least one of four RHD specific polymorphism tested (promoter, intron 4, exon 7 and 3' UTR). Positive for at least one of three RHD specific polymorphism tested (promoter, intron 4, and 3' UTR).

Wagner et al. BMC Genetics 2001 2:10   doi:10.1186/1471-2156-2-10

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