Table 2

SNP and haplotype pattern of the phenylalanine ammonia-lyase (PAL) encoding gene, assessed by pyrosequencing assays for the SNPs 1, 3, 4 and 5 in a set of 190 European barley cultivars and the 6-rowed mapping parents Steptoe and Morex
Haplotypes SNPs from 5' to 3' Haplotype frequency (No. of cultivars) according row number and growth habit Haplotype frequency (No. of cultivars)
SNP1 SNP3 SNP4 SNP5 2r-S 6r-S 2r-W 6r-W
PAL_H1 A (Pro) C (Leu) A (Arg) G (Ala) 80 38 3 121
PAL_H2 C (Pro) C (Leu) A (Arg) G (Ala) 13 1 3 25 42
PAL_H3 C (Pro) T (Leu) T (Arg) A (Ala) 1 1 20 6 28
Unknown Missing SNP information 1 1
Total 94 2 61 35 192

All SNP markers shown in 5' to 3' direction are localized in the exonic region. Coding amino acids are given in brackets. S = Spring, W = Winter, 2r = two-rowed, 6r = six-rowed.

Peukert et al.

Peukert et al. BMC Genetics 2013 14:97   doi:10.1186/1471-2156-14-97

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