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Open Access Research article

Signatures of selection identify loci associated with milk yield in sheep

Bianca Moioli*, Maria Carmela Scatà, Roberto Steri, Francesco Napolitano and Gennaro Catillo

Author Affiliations

Consiglio per la Ricerca e la sperimentazione in Agricoltura, via Salaria 31, Monterotondo 00015, Italy

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BMC Genetics 2013, 14:76  doi:10.1186/1471-2156-14-76

Published: 3 September 2013

Abstract

Background

Identification of genomic regions that have been targets of selection for phenotypic traits is one of the most challenging areas of research in animal genetics, particularly in livestock where few annotated genes are available. In this study a genome-wide scan using the Illumina SNP50K Beadchip was performed in the attempt to identify genomic regions associated with milk productivity in sheep. The ovine genomic regions encoding putative candidate genes were compared with the corresponding areas in Bos taurus, as the taurine genome is better annotated.

Results

A total of 100 dairy sheep were genotyped on the Illumina OvineSNP50K Beadchip. The Fisher’s exact test of significance of differences of allele frequency between each pair of the two tails of the distribution of top/worse milk yielders was performed for each marker. The genomic regions where highly divergent milk yielders showed different allele frequencies at consecutive markers was extracted from the OAR v3.1 Ovine (Texel) Genome Assembly, and was compared to the corresponding areas in Bos taurus, allowing the detection of two genes, the Palmdelphin and the Ring finger protein 145. These genes encoded non-synonymous mutations correlated with the marker alleles.

Conclusion

The innovation of this study was to show that the DNA genotyping with the Illumina SNP50K Beadchip allowed to detect genes, and mutations in the genes, which have not yet been annotated in the livestock under investigation.

Keywords:
OvineSNP50K Beadchip; Dairy sheep; Candidate genes