High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI
- Equal contributors
1 Science & Technology Department, BGI-Shenzhen, Building No. 11, Bei Shan Industrial Zone, Yantian District, Shenzhen 518083, China
2 College of Life Sciences, Wuhan University, Luojia Road No.16, Wuchang District, Wuhan 430072, China
3 School of Life Science and Technology, University of Electronic Science and Technology of China, No.4, Section 2, North Jianshe Road, Chengdu 610054, China
BMC Genetics 2013, 14:56 doi:10.1186/1471-2156-14-56Published: 18 June 2013
As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection.
In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method.
According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.