Open Access Research article

Genetic variation in polyploid forage grass: Assessing the molecular genetic variability in the Paspalum genus

Fernanda W Cidade1, Bianca BZ Vigna2, Francisco HD de Souza2, José Francisco M Valls3, Miguel Dall’Agnol4, Maria I Zucchi5, Tatiana T de Souza-Chies6 and Anete P Souza17*

Author Affiliations

1 Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), CP 6010, Campinas, SP CEP 13083-875, Brazil

2 Brazilian Agricultural Research Corporation (Embrapa) Southeast Livestock, CP 339, São Carlos, SP CEP 13560-970, Brazil

3 Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica - PqEB, CP 02372, Brasília, DF CEP 70770-917, Brasil

4 Faculty of Agronomy, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 7712 Agronomia, Porto Alegre, Rio Grande do Sul CEP 91501-970, Brazil

5 Agência Paulista de Tecnologia dos Agronegócios/APTA, Km 30, CP 28, Pólo Regional Centro Sul, Rodovia SP127, Piracicaba, SP CEP13400-970, Brazil

6 Department of Botany, Prédio 43433, Federal University of Rio Grande do Sul, Av. Bento Gonçalves, 9500 Agronomia, Porto Alegre, Rio Grande do Sul, CEP 91501-970, Brazil

7 Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), CP 6109 Campinas, SP, CEP 13083-875, Brazil

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BMC Genetics 2013, 14:50  doi:10.1186/1471-2156-14-50

Published: 8 June 2013

Additional files

Additional file 1:

Accessions from thePaspalumcollection used in the whole germplasm analysis. Accessions from the Paspalum collection used in the whole germplasm analysis. The ID, BRA code, species, botanical groups, origin, chromosome numbers and the reference for the chromosome number determination are shown [23,31,32,114,122-126].

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Additional file 2:

Paspalum notatumaccessions evaluated in this study.Paspalum notatum accessions evaluated in this study. The CODE used in the figures, ID, collector identification, BRA CODE, site of origin, chromosome number, ploidy level, geographic coordinates and availability of morphological data are shown.

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Additional file 3:

Chromosome number, polyploidy type, reproduction mode and genomes found in thePaspalumspecies. Chromosome number, type of polyploidy, reproduction mode and genomes found in the Paspalum species based on the literature [17,27,30-32,102-104,106-108,110,113,114],[122,124,125,127-157].

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Additional file 4:

Size range, number of bands and PIC from the 12 SSR loci transferred to thePaspalumtaxa. Size range, number of bands and PIC from the 12 SSR loci transferred to the Paspalum taxa.

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Additional file 5:

Magnitude ofΔK from STRUCTURE analysis of the germplasm. Magnitude of ΔK from STRUCTURE analysis of K (arithmetic mean ± s.d. over 10 replicates) calculated following the ΔK method proposed by Evanno et al. [84] for Paspalum microsatellite data. The modal values of these distributions indicate that the true K, or the uppermost level of the STRUCTURE analysis, is seven genetic groups.

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Additional file 6:

Magnitude ofΔK from the STRUCTURE analysis of thePaspalum notatumaccessions. Magnitude of ΔK from the STRUCTURE analysis of K (mean ± SD over 8 replicates), calculated following the ΔK method proposed by Evanno et al. [84] for Paspalum notatum microsatellite data.

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Additional file 7:

Mean probabilities for each population from the STRUCTURE analysis for K = 7 forPaspalum notatum. Mean probabilities for each population from the STRUCTURE analysis for K = 7 for Paspalum notatum.

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Additional file 8:

Statistics describing nine morphological traits from 46Paspalum notatumaccessions. Statistics describing nine morphological traits from 46 Paspalum notatum accessions.

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Additional file 9:

Principal components of nine morphological traits from 46Paspalum notatumaccessions. Principal components of nine morphological traits from 46 Paspalum notatum accessions.

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Additional file 10:

Botanical identification of Paspalum notatumaccessions according to different authors and the corresponding STRUCTURE group. Botanical identification of Paspalum notatum accessions according to different authors and the corresponding STRUCTURE group.

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Additional file 11:

Map of the geographic distribution of 57 of thePaspalum notatumaccessions analyzed. Geographic distribution of the 57 Paspalum notatum accessions that were analyzed genotypically and phenotypically. Point colors correspond to the colors in the STRUCTURE clusters from Figure 5. A sole accession from Florida, USA, has not been represented in the map.

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