Open Access Highly Accessed Research article

Molecular diversity and population structure at the Cytochrome P450 3A5 gene in Africa

Ripudaman K Bains1*, Mirna Kovacevic12, Christopher A Plaster1, Ayele Tarekegn3, Endashaw Bekele3, Neil N Bradman4 and Mark G Thomas15

Author Affiliations

1 Research Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London, WC1E 6BT, UK

2 Centre for Mathematics and Physics in the Life Sciences and Experimental Biology (CoMPLEX), University College London, Physics Building, Gower Street, London, WC1E 6BT, UK

3 Addis Ababa University and Center of Human Genetic Diversity, P.O. Box 1176, Addis Ababa, Ethiopia

4 Henry Stewart Group, 28/30 Little Russell Street, London, WC1A 2HN, UK

5 Department of Evolutionary Biology, Uppsala University, Uppsala, Sweden

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BMC Genetics 2013, 14:34  doi:10.1186/1471-2156-14-34

Published: 3 May 2013

Additional files

Additional file 1:

Table S1.“The proportion of each inferred CYP3A5 haplotype observed in each population.” The Table lists the frequencies of each inferred CYP3A5 haplotype, by population.

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Additional file 2:

Figure S1. “The distribution of high-, intermediate- and low- CYP3A5 expression phenotypes, inferred from diplotypes.” The Figure shows inferred CYP3A5 expression phenotypes, assuming that CYP3A5*6 causes low/non-expression of CYP3A5. The size of each circle is proportional to the number of individuals sampled from a given population (see Additional file Table S1).

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Additional file 3:

Figure S2. “The distribution of high-, intermediate- and low- CYP3A5 expression phenotypes, inferred from diplotypes.” The Figure shows inferred CYP3A5 expression phenotypes, assuming that CYP3A5*6 does not cause low/non-expression of CYP3A5. The size of each circle is proportional to the number of individuals sampled from a given population (see Additional file Table S1).

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Additional file 4:

Figures S3a and b. “Haplotypes inferred from genotype data in 8 populations.” Supplementary Figure 3a shows the composition of each CYP3A5 haplotype inferred from genotype data for 8 global populations. The frequencies of each haplotype, by population, are shown in Additional file Figure S3b.

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Additional file 5:

Table S2. “Geographic co-ordinates, sample size and major language family of each population genotyped in the geographic survey of clinically relevant CYP3A5 alleles. The CYP3A5 gene was re-sequenced in five Ethiopian populations.” This Table provides details of all populations which were genotyped, and re-sequenced for this study.

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Additional file 6:

Table S3. “A list of the primers used for PCR amplification and sequencing of CYP3A5.”

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