PAR boundary. Determination of the PAR boundary. A) Log2 plot as per Figure 1, focussed on the PAR boundary region. The black line shows the 5-clone average of log2 ratios. Red and green lines show the approximate region encompassed by individual BACs in the tiling path, at appropriate log2 positions. Red lines show BACs confirmed as XY-homologous by FISH; green lines show X-only BACs. The PAR boundary must lie within the highlighted ~400 kb region of 6.5 Mb to 6.9 Mb. B) FISH image of the last PAR BAC (CH242-236H7). C) FISH image of the subsequent X-specific BAC (CH242-156O11).
Skinner et al. BMC Genetics 2013 14:3 doi:10.1186/1471-2156-14-3