Electropherograms of the MYH7 c. 4320_4321insCCCGCC mutation. Representative sequence traces of PCR products amplified from genomic DNA of 3 pigs with the different genotypes are shown on the right hand side. The presence of the mutation can be directly visualized by fragment size analysis of a fluorescently labeled PCR product containing the mutation as shown on the left. Note that the mutant allele with the 6 bp-insertion is present in heterozygous form in ‘Campus’ and in CPS affected piglets at different ratio.
Murgiano et al. BMC Genetics 2012 13:99 doi:10.1186/1471-2156-13-99