Seed germination and plant cultivation. A. Barley seeds were germinated in the dark at room temperature. B. After 72 h, seedlings that had three to five roots of 1.5 to 2.0 cm length and that had not yet started to extend the shoot were selected. Each seedling was inserted into a 1.5 ml reaction tube (Eppendorf, Hamburg) that was cropped at the bottom. C. The tubes with the seedlings were subsequently plugged into the perforated trays and placed on top of the boxes. D. Throughout the experiments the roots plunged into the nutrient solution whereas plant shoots grew on top of the tray.
Hoffmann et al. BMC Genetics 2012 13:88 doi:10.1186/1471-2156-13-88