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Open Access Highly Accessed Research article

Trypanotolerance in N’Dama x Boran crosses under natural trypanosome challenge: effect of test-year environment, gender, and breed composition

Caleb O Orenge12*, Leonard Munga2, Charles N Kimwele3, Steve Kemp45, Abraham Korol6, John P Gibson7, Olivier Hanotte8 and Morris Soller9

Author Affiliations

1 South Eastern University College (A constituent college of the University of Nairobi), P.O. Box 170–90200, Kitui, Kenya

2 Kenya Agricultural Research Institute - Trypanosomiasis Research Centre (KARI-TRC), P.O. Box 362, Kikuyu, Kenya

3 Department of Veterinary Anatomy and Physiology, University of Nairobi, Nairobi, Kenya

4 School of Biological sciences, University of Liverpool, Liverpool, L69 7ZB, UK

5 International Livestock Research Institute (ILRI), Nairobi, Kenya

6 Department of Evolutionary and Environmental Biology, Institute of Evolution, University of Haifa, Haifa, 31905, Israel

7 The Centre for Genetic Analysis and Applications, University of New England, Armidale, NSW, 235, Australia

8 School of Biology, University of Nottingham, Nottingham, NG7 2RD, UK

9 Department of Genetics, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel

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BMC Genetics 2012, 13:87  doi:10.1186/1471-2156-13-87

Published: 17 October 2012

Abstract

Background

Trypanosomosis, a protozoal disease affecting livestock, transmitted by Glossina (tsetse) flies is a major constraint to agricultural production in Sub-Saharan Africa. It is accepted that utilization of the native trypanotolerance exhibited in some of the African cattle breeds to improve trypanotolerance of more productive but susceptible breeds, will offer a cost effective and sustainable solution to the problem. The success of this approach is based on the premise that quantitative trait loci previously identified under relatively controlled situations confer useful trypanotolerance under natural field situations. As part of a study to authenticate this hypothesis, a population of 192 cattle, consisting of six batches of N’Dama and Kenya-Boran backcross animals [(N’Dama x Kenya-Boran) x Kenya-Boran] born over the period 2002 to 2006 was constructed. Some of the batches also included pure Kenya-Boran cattle, or N’Dama x Kenya- Boran F1 animals. Each batch was exposed as yearlings to natural field trypanosomosis challenge over a period of about one year; the entire challenge period extending from December 2003 to June 2007. Performance of the animals was evaluated by weekly or biweekly measurements of body weight, packed blood cell volume (PCV), parasitemia score, and number of trypanocide treatments. From these basic data, 49 phenotypes were constructed reflecting dynamics of body weight, packed cell volume (PCV) and parasitemia under challenge.

Results

Females were distinctly more trypanotolerant than males. F1, backcross and pure Kenya- Boran animals ranked in that order with respect to trypanotolerance. Overall batch effects were highly significant (p<0.001) for most traits, and were generally more significant than the gender or genetic type effects. The superior trypanotolerance of the F1 animals was expressed in all three components of animal defense strategies against pathogens: Avoidance resistance, and tolerance.

Conclusions

The results show that trypanotolerance derived from the N’Dama is expressed under field conditions; and that the trait is primarily additive in nature, being expressed in heterozygous condition and in a three-quarters Boran genetic background. The results further, underscore the complexity of the trait in the field manifesting all three host disease-control strategies, and show the importance of gender and local environmental conditions in determining response to challenge.

Keywords:
Trypanotolerance; Tsetse challenge; N’Dama; Boran; N’Dama x Boran crosses; Gender effect; Sub-Saharan Africa