Genomic DNA and cDNA evidence for the 13 WC1 genes in two bovine breeds. PCR analysis was conducted by using genomic DNA and cDNA derived from cattle of two different breeds (Belted Galloway, designed with prefixes of “Y_gDNA and Y_cDNA”, respectively; Holstein, designed with prefixes of “B_gDNA and B_cDNA”, respectively). Primer pairs for distinguishing thirteen WC1 domain 1s were thirteen WC1 specific primer sets. For each primer set, the identities of the amplified products were confirmed by DNA sequencing analysis.
Chen et al. BMC Genetics 2012 13:86 doi:10.1186/1471-2156-13-86