Specific amplification of each WC1 Domain 1. (A) Primer specificities of thirteen WC1 specific primer sets. Confirmation of PCR amplification specificities by gel electrophoresis for thirteen WC1 specific primer sets (WC1-1, WC1-3, WC1-4, WC1-5, WC1-6, WC1-7, WC1-9, WC1-10, WC1-11, WC1-12, WC1-13, WC1-nd1, and WC1-nd2) and the bovine IFNB primer pair. Plasmids containing thirteen WC1 domain 1 gene sequences were used as templates in PCR reactions. For each primer set, the identities of the amplified products were confirmed by DNA sequencing analysis. (B) Genomic DNA and cDNA evidence for Dominette. PCR analysis was conducted by using genomic DNA and cDNA derived from the reference animal Dominette of the Hereford breed (designed with prefixes of “D_gDNA and D_cDNA”, respectively). Primer pairs for amplification of thirteen WC1 SRCR domain 1 sequences with the specific primer sets tested in (A). For each primer set, the identities of the amplified products were confirmed by DNA sequencing analysis.
Chen et al. BMC Genetics 2012 13:86 doi:10.1186/1471-2156-13-86