Table 1

Body and fat pad weights of B6.TH-tabw2 congenic, subcongenic, and B6-homozygous control mice on chow and high fat-high sucrose diets (HFS) (males)
TH genome retained (Mb) Body weight (g) Fat pad weight (g)
Group Maximum Minimum Chow HFS Chow HFS
Control 0 0 28.7 ± 0.4a (n = 15) 29.6 ± 0.7d (n = 17) 0.81 ± 0.06c (n = 15) 1.77 ± 0.27c (n = 17)
tabw2 140.1 112.3 28.4 ± 0.5ab (n = 13) 36.9 ± 1.1a (n = 7) 1.15 ± 0.07a (n = 13) 5.22 ± 0.42a (n = 7)
Line A 57.9 39.9 27.4 ± 0.4bc (n = 19) 29.8 ± 0.9cd (n = 11) 0.85 ± 0.06bc (n = 19) 2.87 ± 0.33b (n = 11)
Line B 92.5 57.2 27.2 ± 0.5bc (n = 11) 31.3 ± 0.8bcd (n = 14) 1.16 ± 0.07a (n = 11) 2.74 ± 0.30b (n = 14)
Line C 45.1 30.7 26.9 ± 0.3c (n = 29) 32.2 ± 0.6b (n = 28) 0.99 ± 0.05ab (n = 29) 2.70 ± 0.21b (n = 28)
Line D 10.8 1.4 28.5 ± 0.3a (n = 33) 31.7 ± 0.5bc (n = 40) 0.95 ± 0.04bc (n = 33) 2.49 ± 0.18b (n = 40)
ANOVA p-value 0.0007 <0.0001 0.0003 <0.0001

The maximal TH interval retained is marked by the most proximal and distal primers with known B6 homozygous genotypes for a given donor region. The minimal TH interval retained is marked by the most proximal and distal primers with known TH homozygous genotypes for a given donor region. Fat pad weight is the sum of five fat pad weights (inguinal, epididymal, mesenteric, retroperitoneal including perirenal, and subscapular). Data are means ± SEM. Group means labeled with different letters are significantly different within a diet (P < 0.05)

Stewart et al.

Stewart et al. BMC Genetics 2012 13:81   doi:10.1186/1471-2156-13-81

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