ssl1-1mutation affects nuclear accumulation of poly(A)+RNA inmex67-5mutant cells. Single mex67-5 and double mex67-5 ssl1-1 [either transformed with the empty vector (vector) or SSL1 in a CEN plasmid (pSSL1)] mutant cells were grown to mid-log phase at 23 °C and incubated for 1 hour at 37 °C. In all the cases, cells were fixed and in situ hybridization was performed using a digoxigenin-conjugated oligo(dT) probe, followed by incubation with a FITC-conjugated anti-digoxigenin antibody [poly(A)]. DNA was visualized by DAPI staining.
Estruch et al. BMC Genetics 2012 13:80 doi:10.1186/1471-2156-13-80