Figure 4.

Suppression ofmex67ts growth defect byssl1-1. (A) rat8-2, yra1-1, mex67-5 and sub2-85 mutations were combined with ssl1-1. Double mutant strains were transformed with a CEN plasmid containing the SSL1 wild type gene (pSSL1), or the empty vector pRS316 (vector). Serial dilutions (1:10) were spotted onto SC-ura plates and incubated for 3 days at different temperatures. (B) Suppression of mex67-6 ts growth defect by ssl1-1. Serial dilutions (1:10) of mex67-6 single mutant and mex67-6 ssl1-1 double mutant (either transformed with pSSL1 or the empty vector pRS316) were spotted onto YPD plates and incubated for 3 days at different temperatures. Effect of the copy number of the SSL1 wild type gene on growth of the mex67-5 ssl1-1 double mutant strain. Serial dilutions (1:10) of mex67-5 single mutant and mex67-5 ssl1-1 double mutant transformed with SSL1 in a CEN/URA3 plasmid (pSSL1) or in a multicopy vector (pPD5) were spotted onto YPD plates and incubated for 3 days at different temperatures.

Estruch et al. BMC Genetics 2012 13:80   doi:10.1186/1471-2156-13-80
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