Table 5

Summary of quality control steps and overall assay performance for the telomere (T), reference (S), and telomere length estimates (T/S)
Criteria Target Assay I Assay II Assay III Assay IV Figure/Table
Serial dilution series
LDR T Borderline Within range Within range Within range Figure 2
S Borderline Within range Within range Within range
NTC T OK Borderline Borderline OK Figures 3, 4
S OK Overlap* Overlap Overlap*
Magnitude E T Intermediate Intermediate Intermediate High Figure 1
S Intermediate Low Intermediate Intermediate
Interbatch E T Constant Variable Constant Constant Figure 1
S Constant Variable Constant Constant
Dilution E T Variable E No change No change No change Table 3
S Variable E No change No change No change
Precision T Intermediate High High High Figure 5, Table 4
S Intermediate Low Moderate High
Accuracy T High High High High Table 4
S High Intermediate High Intermediate
Resolution T Low High High High Table 5
S Intermediate Intermediate High High
Precision T/S Intermediate Low Intermediate Intermediate Additional file 3: Figure S2
Accuracy T/S Low Low High High Additional file 3: Figure S2
"Unknown" samples
Outliers T/S Few Many Many Few Additional file 1: Table S1
Resolution T/S Intermediate Low Low High Additional file 1: Table S1
Quantitative method T/S High Low Intermediate High Additional file 5: Table S2
Inter-assay correlation T/S Low Low Low Intermediate Additional file 4: Figure S3
Assay evaluation Intermediate Worst Intermediate Best

Olsen et al.

Olsen et al. BMC Genetics 2012 13:77   doi:10.1186/1471-2156-13-77

Open Data