Table 2

Precision and accuracy of the telomere and reference gene reactions quantified in terms of the standard deviation of Cq values (Cq SD), as well as the correlation coefficient (R2) and the slope of the regression line between expected and observed amount log DNA as illustrated in Figure1
Pfaffl Ruijter
Target Assay Cq SD R2 Slope SD R2 Slope SD
Telomere I 0.330 95.6 1.00 0.021 95.8 1.00 0.021
II 0.171 98.5 0.99 0.019 99.0 0.99 0.016
III 0.115 99.4 1.00 0.012 99.4 1.00 0.012
IV 0.116 99.2 1.00 0.012 99.5 1.01 0.010
Reference I 0.423 95.7 1.06 0.027 95.6 1.00 0.021
II 0.476 92.0 0.98 0.045 93.4 1.02 0.044
III 0.264 97.0 0.99 0.027 93.4 0.97 0.039
IV 0.196 96.4 0.97 0.033 97.6 1.02 0.022

Olsen et al.

Olsen et al. BMC Genetics 2012 13:77   doi:10.1186/1471-2156-13-77

Open Data