Table 1

Experimental setup of the four qPCR assays for relative telomere length estimation
Reaction conditions Primers Standards Telomere Reference
Assay Platform Type Master mix Duration Tel Ref Batches Dilutions LDR Cq NTC Cq NTC
I ABI Singleplex Commercial 21 CW2006 BA 1998 7 5 4.5-72.0 17.6 34.9 33.5 40.0
II RG Multiplex RC 2009 6 RC 2009 RC 2009 4 4 2.2-60.0 15.7 19.4 39.9 27.9
III RG Singleplex RC 2009 5 RC 2009 RC 2009 4 4 2.2-60.0 14.1 15.5 37.4 36.2
IV RG Multiplex Commercial 6 RC 2009 RC 2009 4 5 4.5-72.0 13.9 27.2 34.3 31.1

Duration = number of days it took to generate the data once assays had been optimized; BA = Bérubé and Aguilar [38]; CW 2006 = Callicot and Womack [52]; RC 2009 = Cawthon [24]; Batches = number of separate qPCR runs (plates); Dilutions = number of dilution steps; LDR = linear dynamic range (ng/reaction); Cq = the average Cq of the serially diluted standard with the lowest amount of DNA; NTC = the lowest Cq value observed for a NTC.

Olsen et al.

Olsen et al. BMC Genetics 2012 13:77   doi:10.1186/1471-2156-13-77

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