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Open Access Research article

Heterogeneous evolutionary rates of Pi2/9 homologs in rice

Kejing Wu, Ting Xu, Changjiang Guo, Xiaohui Zhang* and Sihai Yang*

Author Affiliations

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, China

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BMC Genetics 2012, 13:73  doi:10.1186/1471-2156-13-73

Published: 19 August 2012

Additional files

Additional file 1:

Figure S1. Phylogenetic tree derived from NBS domains of homologs in completely and partially sequenced Pi2/9 locus. To further confirm the topological relationships in Figure 1, another NJ tree was constructed by including some additional NBS-LRR genes from partially sequenced Pi2/9 locus by PCR amplification or BAC-end sequence based homolog searches. (PDF 92 kb)

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Additional file 2:

Figure S2. Gene collinearity in orthologous regions between rice and sorghum. One syntenic region pair was detected between rice and sorghum genome. Each gene was indicated as horizontal lines. Orthologous genes were joined by solid lines. The genes marked by red lines represented Pi2/9 homologs. (PDF 32 kb)

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Additional file 3:

Table S1. The distribution of Pi2/9 homologs corresponding to Figure 1. The gene copy number variations of Pi2/9 homologs were found in subfamilies. Subfamilies 1–10 referred to corresponding phylogenetic tree in Figure 1, which was derived from all Pi2/9 homologs in completely and partially sequenced Pi2/9 locus. Table S2 as XLS Sequences of oligonucleotide primers used in PCR amplification. Locus-specific primers were designed based on conserved sites of the consensus NBS domain to investigate Pi2/9 homolog variation in four cultivars, Q2436, Tadukan, GM2 and Tetep. (XLS 30 kb)

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