Gene variation at the CCL8 locus within and among leporids. PCR products of leporid specimens were obtained using either gDNA or cDNA and are aligned with the CCL8ps sequence of rabbit WGS. Potential Stop and Translation Initiating codons are underlined. The positions of the cDNA primers are bold underlined. ‘Orcu CCL8ps’ represents the consensus of 11 haplotypes of Oryctolagus cuniculus specimens of both subspecies (O. c. cuniculus and O. c. algirus), after excluding one haplotype that was identical to CCL8ps of WGS (OccTar109allele1) and ignoring singletons (i.e. nucleotide differences observed only once). Occ: Oryctolagus cuniculus; Bumo: Bunolagus monticularis; Syfl: Sylvilagus floridanus; Leti: Lepus timidus; Legr: Lepus granatensis; Leeu: Lepus europaeus. ‘.’: identical to leader sequence; ‘-‘: indel. The data shown are limited to the exon containing fractions. An alignment of the entire 1.6Kb gene region is presented in Additional file 7.
van der Loo et al. BMC Genetics 2012 13:72 doi:10.1186/1471-2156-13-72