Open Access Highly Accessed Research article

Genome-wide mRNA sequencing of a single canine cerebellar cortical degeneration case leads to the identification of a disease associated SPTBN2 mutation

Oliver P Forman1*, Luisa De Risio2, Jennifer Stewart3, Cathryn S Mellersh1 and Elsa Beltran2

Author Affiliations

1 Kennel Club Genetics Centre, Animal Health Trust, Kentford, Newmarket, Suffolk, CB8 7UU, UK

2 Neurology Department, Animal Health Trust, Kentford, Newmarket, Suffolk, CB8 7UU, UK

3 Centre for Preventive Medicine, Animal Health Trust, Kentford, Newmarket, Suffolk, CB8 7UU, UK

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BMC Genetics 2012, 13:55  doi:10.1186/1471-2156-13-55

Published: 10 July 2012

Additional files

Additional file 1:

Primers used for qPCR assays of theSPTBN2,ACTB and TBPgenes. All probes were 5’ 6-FAM and 3’ Iowa Black labelled, with internal ZEN labelling.

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Additional file 2:

Summary of candidate genes investigated for sequence polymorphisms after mRNA-seq of a single NCCD Beagle case.

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Additional file 3:

Relative expression analysis data. Expression levels of SPTBN2 were measured relative to ACTB and TBP using qPCR. Fold change was calculated based on changes in threshold cycle (Ct) measurements within (ΔCt) and between (ΔΔCt) the case and control.

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