Differentially represented organisms in rumen fluid and faeces. The number of sequence read alignments to each contig in three databases were compared between faeces and rumen fluid using a t-test. BLASTn was then used to assign contigs that were significantly differently represented (p < 0.001) between rumen fluid and faeces to a taxon. The BLAST output was displayed using MEGAN.
Ross et al. BMC Genetics 2012 13:53 doi:10.1186/1471-2156-13-53