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Open Access Highly Accessed Methodology article

High throughput whole rumen metagenome profiling using untargeted massively parallel sequencing

Elizabeth M Ross123*, Peter J Moate4, Carolyn R Bath12, Sophie E Davidson1, Tim I Sawbridge123, Kathryn M Guthridge12, Ben G Cocks123 and Ben J Hayes123

Author Affiliations

1 Biosciences Research Division, Department of Primary Industries, Bundoora, VIC, 3083, Australia

2 Dairy Futures Cooperative Research Centre, Bundoora, VIC, 3083, Australia

3 La Trobe University, Bundoora, VIC, 3086, Australia

4 Department of Primary Industries, Ellinbank Centre, Ellinbank, VIC, 3820, Australia

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BMC Genetics 2012, 13:53  doi:10.1186/1471-2156-13-53

Published: 2 July 2012

Additional files

Additional file 1:

Contains a detailed account of sampling for the DPI_rumen database, a summary of the six databases used including size and number of contigs, a BLAST analysis of the rumen samples, details of the databases used for hierarchical clustering and hierarchical clustering results using a Binary (presence/absence) distance matrix method [[15,16,22,31],[34],[35]].

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