RPA foci in the suppressor strains. Cells with RPA foci were identified using GFP fused to Rpa1 as described . A) An example of RPA foci is shown. Bright field (BF) and fluorescent (GFP) images are shown for MCM2 (left panels) and srs2Δ mcm2AA (right panels). A RPA focus in the srs2Δ mcm2AA image is indicated by the white arrow. B) The percentage of cells containing RFA foci was measured in triplicate experiments and standard deviation was determined. The asterisks indicate strains that do not have statistically significant different frequency of RPA foci compared to mcm2AA (p ≥ 0.05, Student’s unpaired t-test) while the crosses indicate strains that are statistically different from wild type (p < 0.05).
Stead et al. BMC Genetics 2012 13:36 doi:10.1186/1471-2156-13-36