Table 1

Yeast strains

Strain number

Description

TRA1 plasmid

Reference


CY1021

Isogenic to KY320 except tra1Δ

YCplac22-myc-TRA1

[2]

CY2706

Isogenic to CY1021

YCplac22-myc9-TRA1

[23]

CY3003

Isogenic to CY1021

YCplac22-myc9-tra1-L3733A

[23]

CY4018

Isogenic to CY1021

YCplac22u-myc9-tra1-L3733A

This study

CY5522

Isogenic to CY3003 except MATα

YCplac22-myc9-tra1-L3733A

This study

CY5557

Diploid cross of CY2706 and CY4413

YCplac22-myc9-TRA1

This study

CY5558

Diploid cross of CY4018 and CY5522

YCplac22-myc9-tra1-L3733A

This study

CY5579

isogenic to CY4018 except es2 (upf11-164)

YCplac22u-myc9-tra1-L3733A

This study

CY5580

isogenic to CY4018 except es12

YCplac22u myc9-tra1- L3733A

This study

CY5581

isogenic to CY4018 except es35

YCplac22u myc9-tra1- L3733A

This study

CY5582

isogenic to CY4018 except es36

YCplac22u myc9-tra1- L3733A

This study

CY5583

isogenic to CY4018 except es37

YCplac22u myc9-tra1- L3733A

This study

CY5584

isogenic to CY4018 except es38

YCplac22u myc9-tra1- L3733A

This study

CY5585

isogenic to CY4018 except es39

YCplac22u-myc9-tra1-L3733A

This study

CY5586

isogenic to CY4018 except es40

YCplac22u-myc9-tra1-L3733A

This study

CY5587

isogenic to CY4018 except es41

YCplac22u-myc9-tra1- L3733A

This study

CY5588

isogenic to CY4018 except es42

YCplac22u-myc9-tra1-L3733A

This study

CY5750

isogenic to CY4018 except es43

YCplac22u-myc9-tra1-L3733A

This study

CY5666

Isogenic to CY5522 except es2 (upf11-164)

YCplac22-myc9-tra1-L3733A

This study

CY5758

Isogenic to CY5522 except es38

YCplac22-myc9-tra1-L3733A

This study

CY5603

Isogenic to CY5522 except es41

YCplac22-myc9-tra1-L3733A

This study

CY5688

Isogenic to CY2706 except es2 (upf11-164)

YCplac22u-myc9-TRA1

This study

CY5690

Isogenic to CY2706 except es38

YCplac22u-myc9-TRA1

This study

CY5691

Isogenic to CY2706 except es41

YCplac22u-myc9-TRA1

This study

KY320

MATa ura3-52 ade2-101 trp1-Δ1 lys2-801 his3-Δ200 leu2::PET56

[66]

CY4413

MATα ura3-52 ade2-101 trp1-Δ1 lys2-801 his3-Δ200 leu2::PET56

CY2222

MATa can1Δ::STE2pr-SpHIS5 lyp1Δ his3Δ 1 leu2Δ0 ura3Δ0 met10 LYS2+ TRA1-SRR3413-URA3

[69]

BY4741

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

[70]

BY4742

MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0

[70]

BY2940

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 eaf7::KanMX

[70]

BY4282

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ada2::KanMX

[70]

BY7143

MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 eaf3::KanMX

[70]

BY41905

MATa/α his3Δ1/his3Δ1 leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0 + can1Δ::LEU2 + -MFA1pr-HIS3/CAN1+ upf2Δ::KanMX

[71]

BY44702

MATa/α his3Δ1/his3Δ1 leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0 can1Δ::LEU2 + -MFA1pr-HIS3/CAN1+ upf3Δ::KanMX

[71]

BY46214

MATa/α his3Δ1/his3Δ1 leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0 can1Δ::LEU2 + -MFA1pr-HIS3/CAN1+ upf1Δ::KanMX

[71]

CY4350

MATa ura3Δ0 his3Δ0 leu2Δ0 TRA1-F3744A-HIS3

[23]

CY4353

MATa ura3Δ0 his3Δ0 leu2Δ0 TRA1-HIS3

[23]

CY4103

MATa ura3Δ0 his3Δ0 leu2Δ0 TRA1-L3733A-HIS3

[23]

CY5932

MATa his3Δ1 leu2Δ0 ura3Δ0 upf1Δ::KanMX

This study

CY5934

MATa his3Δ1 leu2Δ0 ura3Δ0 upf2Δ::KanMX

This study

CY5936

MATa his3Δ1 leu2Δ0 ura3Δ0 upf3Δ::KanMX

This study

CY5937

MATα his3Δ1 leu2Δ0 ura3Δ0 upf3Δ::KanMX

This study

CY5938

MATa his3Δ1 leu2Δ0 ura3Δ0 upf1Δ::nat1

This study

CY5939

MATα his3Δ1 leu2Δ0 ura3Δ0 upf1Δ::nat1

This study

CY5940

MATα ura3Δ0 his3Δ0 leu2Δ0 URA3-Flag5-TRA1

This study

CY5967

MATα ura3Δ0 his3Δ0 leu2Δ0 tra1-L3733A-HIS3

This study

CY5968

MATα his3Δ1 leu2Δ0 ura3Δ0 upf1Δ::KanMX

This study

CY5972

MATa ura3Δ0 his3Δ0 leu2Δ0 tra1-L3733A-HIS3 upf1Δ::KanMX

This study

CY5976

MATα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 eaf7::KanMX upf1Δ::nat1

This study

CY5979

MATα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ada2::KanMX upf1Δ::nat1

This study

CY5980

MATα his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 eaf3::KanMX upf1Δ::nat1

This study

CY5983

MATa ura3Δ0 his3Δ0 leu2Δ0 tra1-L3733A-HIS3 upf3Δ::KanMX

This study

CY5996

MATa ura3Δ0 his3Δ0 leu2Δ0 tra1-L3733A-HIS3 upf2Δ::KanMX

This study

CY6004

MATa ura3Δ0 his3Δ0 leu2Δ0 URA3-Flag5-tra1-L3733A-HIS3

This study

CY6005

MATa ura3Δ0 his3Δ0 leu2Δ0 URA3-Flag5-tra1-L3733A-HIS3 upf1Δ::KanMX

This study

CY6030

MATa ura3Δ0 his3Δ0 leu2Δ0 tra1-F3744A-HIS3 upf1Δ::KanMX

This study

CY6102

MATα ura3Δ0 leu2Δ0 tra1-SRR3413-URA3 upf1Δ::KanMX

This study


Kvas et al. BMC Genetics 2012 13:19   doi:10.1186/1471-2156-13-19

Open Data