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Open Access Research article

Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour

Jadwiga Śliwka1*, Henryka Jakuczun1, Marcin Chmielarz1, Agnieszka Hara-Skrzypiec1, Iga Tomczyńska1, Andrzej Kilian2 and Ewa Zimnoch-Guzowska1

Author affiliations

1 Plant Breeding and Acclimatization Institute-National Research Institute, Młochów Research Centre, Platanowa 19, 05-831 Młochów, Poland

2 Diversity Arrays Technology Pty Ltd., 1 Wilf Crane Cr., Yarralumla, ACT 2600, Australia

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Citation and License

BMC Genetics 2012, 13:11  doi:10.1186/1471-2156-13-11

Published: 27 February 2012

Abstract

Background

Phytophthora infestans (Mont.) de Bary, the causal organism of late blight, is economically the most important pathogen of potato and resistance against it has been one of the primary goals of potato breeding. Some potentially durable, broad-spectrum resistance genes against this disease have been described recently. However, to obtain durable resistance in potato cultivars more genes are needed to be identified to realize strategies such as gene pyramiding or use of genotype mixtures based on diverse genes.

Results

A major resistance gene, Rpi-rzc1, against P. infestans originating from Solanum ruiz-ceballosii was mapped to potato chromosome X using Diversity Array Technology (DArT) and sequence-specific PCR markers. The gene provided high level of resistance in both detached leaflet and tuber slice tests. It was linked, at a distance of 3.4 cM, to violet flower colour most likely controlled by the previously described F locus. The marker-trait association with the closest marker, violet flower colour, explained 87.1% and 85.7% of variance, respectively, for mean detached leaflet and tuber slice resistance. A genetic linkage map that consisted of 1,603 DArT markers and 48 reference sequence-specific PCR markers of known chromosomal localization with a total map length of 1204.8 cM was constructed.

Conclusions

The Rpi-rzc1 gene described here can be used for breeding potatoes resistant to P. infestans and the breeding process can be expedited using the molecular markers and the phenotypic marker, violet flower colour, identified in this study. Knowledge of the chromosomal localization of Rpi-rzc1 can be useful for design of gene pyramids. The genetic linkage map constructed in this study contained 1,149 newly mapped DArT markers and will be a valuable resource for future mapping projects using this technology in the Solanum genus.

Keywords:
Diversity Array Technology; Mapping; Phytophthora infestans; Solanum tuberosum; Rpi-rzc1