Table 1 |
||||
| Primers used for this study | ||||
| Forward sequence | Reverse sequence | Tm | Size | |
| Nle1 sequencing primers | ||||
| Nle1E1/2 | CTTGACTCCTCCGAACACGAG | AAACACAGCCTGTCTGTAGGTGAG | 62 | 500 |
| Nle1E3 | GATTAAATTTGTCGCATGGTGGTA | GTCTGTTACTTGCAACGTGAGTCC | 62 | 475 |
| Nle1E4 | TATTTCTCCTCAGGGAATGGAGAG | CCACACTCAGTCCAGTATCTGCTT | 62 | 377 |
| Nle1E5/6 | CTGTGTTCTCCCTCACCTCTCC | ATAGTAGGCCAAGCCGTTGCT | 62 | 557 |
| Nle1E7 | ACAGCCTTGCTCTGCTGTTAGAA | GGACCAGCTGGACTCTTGGTATAA | 62 | 440 |
| Nle1E8/9 | TTCCTGATTCTTGCCTTATGTCAC | AACCCTAACTAAGACAACCAAGAACAA | 62 | 544 |
| Nle1E10 | TGGAGTTGCATGTAAGCTTGTGT | GTCACTAGCCCTAAAGATGCCATT | 62 | 488 |
| Nle1E11/12 | CCGGCCCAGGTACCTAGCTT | ACCTACAGGTTCTCCCAGAGTCTCC | 62 | 498 |
| Nle1E13 | ACTTGATACTTGGCAGTAGGCACA | CTCCTGCTATCCAGTGCAAGG | 62 | 570 |
| Nle1 genotyping primers | ||||
| Nle1 GSP | GCTGTAATGTCCTGACTGT | 60 | 637 | |
| cDNA 1 | CTGTGTCGTACTCTTCAAGGTCAT | CTGTGGAGTCATCTTCTCCATATC | ||
| cDNA 2 | TCAGACGACTTCACCTTATTCCTG | CAGTCAACAGCATATACCTCATCG | 62 | 351 |
| Nle1 DNA | TCTCCTTCAGCTCCTTCACTGT | TCCAATGGTGGAGTATAGGGTATAA | 60 | 341 |
| cDNA amplification primers | ||||
| 1st PCR | ATATGGATCCGGCGCGCCGTCGACT24 | ATATCTCGAGGGCGCGCCGGATCCT24 | 67 | |
| 2nd PCR | (NH2)ATATGGATCCGGCGCGCCGTCGACT24 | (NH2)ATATCTCGAGGGCGCGCCGGATCCT24 | 67 | |
| Eed | GTGTGACATTTGGTACATGAGGTT | ACATTTATGATGGGTCAGTGTTGT | 60 | 148 |
| TaqMan gene expression assay | ||||
| Assay ID | Amplicon Size (bp) | |||
| Trp53 | Mm01731290_g1 | 119 | ||
| Cdkn1a | Mm04205640_g1 | 80 | ||
| Gapdh | Mm99999915_g1 | 107 | ||
Lossie et al. BMC Genetics 2012 13:106 doi:10.1186/1471-2156-13-106
