Table 4

PCR primer sequence for candidate QTL-related gene and SNP type used for genotypic analysis
Candidate genes Primer sequence (5'→3') SNP
LpEUI f: ACG TAC CTG TAC TGG CTG C/G
r: TTG CAG TTG TCC ACC ACG AA
LpPDT f: GCA GAA CAA AAA CTC CAA GA n.a. *a
r: TTG GAT CAG CCA TAG ACG CC
LpGA3ox2 f: CGC GCT ACT TCG ACT TCC n.a. *b
r: GAA GAA GGA GGA GAT GGG C
LpDGL1 f: GTT AAC ATT GAT GAA GTT GC A/G
r: ACA CTC TTC TGG ACC TTG GC
LpGA20 f: GGG TGT ACC AGG AGT ACT G n.a. *a
r: TTA CCA TGA AGG TGT CGC CG
LpPh1 f: GCA TTA ATG ATG AAT GGG CT A/G
r: CAT CCA CAC CAG TTA TTC TC
LpPi37 f: CCA GCG GAT ATG CGC AAT CT C/T
r: CAA ATG CTC TCG GCT GAA GG
LpPIPK1 f: GGC CCT TGT AAA TAG TCT CC G/T
r: CCC TTG ACT GTA ATT GGC TC
LpPib f: TCA CGG ATG AGA TCA TGG AC A/T
r: CTG AAG AAG TGT GAT GGA CT
LpD3 f: CCA AGA TGA AAT TGG ACC TC A/G
r: CTG CAT GTC CCG CAA GTT TG
*a no SNP was identified in PCR amplicon.
*b PCR amplicons were not obtained.

Shinozuka et al.

Shinozuka et al. BMC Genetics 2012 13:101   doi:10.1186/1471-2156-13-101

Open Data