Table 1

Strains used in this study

Strain

Genotype

Sources/References


AP22

MATα leu2-3,112 his3-11,15 ura3-52 trp1

[36]

AP22Dchl1

MATα leu2-3,112 his3-11,15 ura3-52 trp1 chl1::HIS3

[36]

8534-8C

MATα his4Δ34 ura3-52 leu2-3,112

[69]

8534-10A

MATa his4Δ34 ura3-52 leu2-3,112

[73]

301-2B

MATα leu2-3,112 hisΔ34 ura3-52 trp1

[66]

PS29-2B

MATa leu2-3,112 his3-11,15 mcm18-1/ctf19

[69,74]a

M29-5D

MATa leu2-3,112 hisΔ34 ura3-52 trp1mcm18-1/ctf19

This study, by crossing 301-2B with PS29-2B

A3

MATa leu2-3,112 his3-11,15

[69]

699

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100

[37]

699Matα

MATα ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100

This study

699Dchl1

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 chl1::HIS3

[37]

SL14

MATa ade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 bar1Δ::LEU2

[37]

SL14Dchl1

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 bar1Δ::LEU2 chl1::HIS3

[37]

US354

MATα leu2 his3 trp1 ade2 ura3 rad53-21

[37]

SL7

MATa leu2 his3 trp1 ade2 ura3 rad53-21

[37]

US3329

MATa leu2::LEU2::tetR-GFP trp1 CEN5::tetOX224::HIS3 ade2-1 ura3 his3

[75]

US3324

MATa ade2-1 leu2-3,112 his 3-11,15 can1-100 scc1-73

Uttam Surana

SL20

MATα ade2-1 can1-100 leu2-3,112 his3-11,15 scc1-73

This study, by crossing US3324 with 699Matα

SL25

MATa leu2::LEU2 tetR-GFP ade2-1 CEN5::tetOX224::HIS3 ura3 scc1-73

This study, by crossing US3329 with SL20

US3329Δchl1

MATa leu2::LEU2:: tetR-GFP ura3 CEN5::tetO X224::HIS3 ade2-1 chl1Δ::TRP1

This study, by deleting CHL1 in US3329

US3329Δmcm17

MATa leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2-1 mcm17Δ::URA3

This study, by deleting MCM17 in US3329

US3329Dmcm21

MATa leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2 mcm21::URA3

This study, by disrupting MCM21 in US3329

US3335

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1

Uttam Surana

US3335Dchl1

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 chl1::HIS3

This study, by disrupting CHL1 in US3335

US3335Δsir3

MATa ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 sir3Δ::HIS3

This study, by deleting SIR3 in US3335


699 and all the strains listed below it are in W303 background, while the strains listed above 699 were from G. Fink.

a The MCM18 gene is the same as CTF19 [74, Saccharomyces Genome Database http://www.yeastgenome.org webcite]. The PS29-2B strain [69] contains the mcm18-1 allele of the MCM18/CTF19 gene.

Laha et al. BMC Genetics 2011 12:83   doi:10.1186/1471-2156-12-83

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