Spindle elongation in ctf4 mutant cells exposed to HU. A and B. Cells of 699 (CTF4) and 699Dctf4 (ctf4) were arrested in G1 phase at 30°C in YEPD medium using α-factor and then released in the same medium at the same temperature in the presence of 0.2 M HU for 3 hours. Thereafter, the cells were processed for spindle staining using anti-α-tubulin antibody. The figure shows wild-type and ctf4 cells having, respectively, short and elongated mitotic spindles. The graphical representation of the distribution of spindle lengths (around 250 for each) is shown in the right panel.
Laha et al. BMC Genetics 2011 12:83 doi:10.1186/1471-2156-12-83