Research article
Functional characterization of the Saccharomyces cerevisiae protein Chl1 reveals the role of sister chromatid cohesion in the maintenance of spindle length during S-phase arrest
1 Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA-01604, USA
2 R&D Manager (Molecular Biology), HiMedia Laboratories Pvt. Ltd., Mumbai, India
3 Molecular Biology & Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore-560 064, India
4 Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VII M, Kolkata
BMC Genetics 2011, 12:83 doi:10.1186/1471-2156-12-83
Published: 23 September 2011Additional files
Additional file 1:
Figure S1. Fields showing split CEN5-GFP dots on the spindle (Y+Y), unsplit CEN5-GFP dots on the spindle (Y) and split or unsplit CEN5-GFP dots not localized on the spindle (G, G+G).
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Additional file 2:
Figure S2. Growth of scc1-73 cells at different temperatures.
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Additional file 3:
Figure S3. DNA content by flow cytometry showing progression of wild-type (SCC1) and mutant (scc1-73) cells after release from G1 arrest at 35°C.
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Additional file 4:
Figure S4. Spot assay for HU sensitivity of US3329 (wild-type), US3329Δchl4 (chl4), US3329Dmcm21 (mcm21) and US3329Δchl1 (chl1) strains.
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