Open Access Research article

Functional characterization of the Saccharomyces cerevisiae protein Chl1 reveals the role of sister chromatid cohesion in the maintenance of spindle length during S-phase arrest

Suparna Laha, Shankar P Das1, Sujata Hajra2, Kaustuv Sanyal3 and Pratima Sinha4*

Author Affiliations

1 Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA-01604, USA

2 R&D Manager (Molecular Biology), HiMedia Laboratories Pvt. Ltd., Mumbai, India

3 Molecular Biology & Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore-560 064, India

4 Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VII M, Kolkata

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BMC Genetics 2011, 12:83  doi:10.1186/1471-2156-12-83

Published: 23 September 2011

Additional files

Additional file 1:

Figure S1. Fields showing split CEN5-GFP dots on the spindle (Y+Y), unsplit CEN5-GFP dots on the spindle (Y) and split or unsplit CEN5-GFP dots not localized on the spindle (G, G+G).

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Additional file 2:

Figure S2. Growth of scc1-73 cells at different temperatures.

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Additional file 3:

Figure S3. DNA content by flow cytometry showing progression of wild-type (SCC1) and mutant (scc1-73) cells after release from G1 arrest at 35°C.

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Additional file 4:

Figure S4. Spot assay for HU sensitivity of US3329 (wild-type), US3329Δchl4 (chl4), US3329Dmcm21 (mcm21) and US3329Δchl1 (chl1) strains.

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