Additional file 1.

Figure S1: Development of microsatellite markers in the 95% confidence interval of the QTL on SSC7. A. A part of the gene map for the human chromosome 14 (from the human genome reference genome assembly of NCBI, Build 37.1). Human sequences in the region corresponding to the QTL on SSC7 were used to search for homologous swine sequences by BLAST analysis. PCR primers for STSs were designed in these swine sequences. B. Swine BAC clones screened with STSs. C. Swine microsatellite markers developed in this study. Microsatellite markers were isolated from the BAC clones by a direct sequencing method using two-nucleotide repeats such as (CA)10 for sequencing primers. D. A part of the SSC7 sequence map (from the swine genome draft sequence, Sscrofa9 assembly, published by the International Swine Genome Sequencing Consortium). The microsatellite markers developed in this study and those on a linkage map developed by Rohrer [28] were assigned to the Sscrofa9 assembly. The underlined markers have not yet been found in the Sscrofa9 assembly. Dotted lines indicate that markers were assigned to multiple positions. E. A part of the SSC7 linkage map for microsatellite markers, developed by Rohrer [28].

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Mikawa et al. BMC Genetics 2011 12:5   doi:10.1186/1471-2156-12-5