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The characterization of a new set of EST-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris

Robertha AV Garcia1, Priscila N Rangel1, Claudio Brondani2, Wellington S Martins3, Leonardo C Melo2, Monalisa S Carneiro4, Tereza CO Borba2 and Rosana PV Brondani2*

Author Affiliations

1 Universidade Federal de Goiás, Escola de Agronomia, CEP 74690-900, Goiânia, GO, Brazil

2 Embrapa Arroz e Feijão, Rodovia GO-462, km 12 Zona Rural, CEP 75375-000, Santo Antônio de Goiás, GO, Brazil

3 Universidade Federal de Goiás, Instituto de Informática, CEP 74690-815, Goiânia, GO, Brazil

4 Universidade Federal de São Carlos, Centro de Ciências Agrárias, Via Anhanguera, km 174, CEP 13600-970, Araras, SP, Brazil

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BMC Genetics 2011, 12:41  doi:10.1186/1471-2156-12-41

Published: 9 May 2011

Additional files

Additional File 1:

Table S1. Characteristics of 302 EST-SSRs markers derived from Phaseolus vulgaris public databases. Primer annealing temperature (AT) that produced scorable PCR products.

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Additional File 2:

Table S2. Cross-amplification of 167 SSR markers on 11 Legume species. The expected allele size (EAS) for each marker is indicated.

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Additional File 3:

Figure S1. Transferability of SSRs across species of the Leguminosae family. The electrophoretic pattern at BM98 (Panel A), PVEST260 (Panel B) and PVEST272 (Panel C) revealed on a polyacrylamide gels across species of the Leguminosae family: 1- Phaseolus vulgaris; 2 - Medicago sativa; 3- P. lunatus; 4- P. coccineus; 5- P. acutifolius; 6- Vigna mungo; 7- V. angularis; 8- V. unguiculata; 9- Glicine max; 10- Arachis hypogaea; 11- Dipteryx alata.

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Additional File 4:

Figure S2. Segregation of informative SSR loci in Phaseolus vulgaris. Polyacrylamide gel resolution and detection by silver staining of SSR PVEST272 (Panel A), PVEST279 (Panel B) and PVEST336 (Panel C) in BAT93 × Jalo EEP558 population. Lane 1 is a 100 bp ladder size standard with the sizes of some fragments indicated in base pairs; lanes 2 and 3 are the two parents, indicated by the arrows, followed by the segregant population.

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